Zhang Hongyan, Wang Shuo
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300222, PR China.
J Immunol Methods. 2009 Oct 31;350(1-2):1-13. doi: 10.1016/j.jim.2009.07.006. Epub 2009 Jul 28.
The current status of enzyme-linked immunosorbent assays (ELISAs) for sulfonamides in edible animal products is reviewed. The attention was focused on the design and synthesis of haptens, conjugation to carrier protein, production of antibody, application of homologous and heterologous systems, as well as the molecular modeling of the haptens and sulfonamides. Researches have shown that sulfonamides seem to be particularly resistant to attempts to produce broad specificity antibodies. By summarizing the available research on sulfonamide ELISAs, it is hoped that it can be considered as a basis for further investigation aimed at developing the most efficient approaches for detection.
本文综述了用于检测食用动物产品中磺胺类药物的酶联免疫吸附测定法（ELISA）的现状。重点关注了半抗原的设计与合成、与载体蛋白的偶联、抗体的制备、同源和异源系统的应用，以及半抗原和磺胺类药物的分子建模。研究表明，磺胺类药物似乎特别难以产生具有广泛特异性的抗体。通过总结磺胺类药物ELISA的现有研究，希望能为进一步研究开发最有效的检测方法提供依据。
