Owan I, Saito A
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukus, Okinawa, Japan.
J Antimicrob Chemother. 1990 Dec;26(6):831-40. doi: 10.1093/jac/26.6.831.
The effect of human urinary colony-stimulating factor (CSF-HU) on in-vitro phagocytosis of Legionella pneumophila, superoxide anion production and intracellular killing of Legion, pneumophila by guinea pig alveolar macrophages was studied. Alveolar macrophages when incubated with CSF-HU demonstrated enhanced phagocytic activity and superoxide production. The bactericidal activity of treated cells was enhanced when they were afterwards incubated with a low concentration of bacteria, but these cells failed to inhibit bacterial multiplication when they were incubated with bacteria in a 1:10 ratio. In vivo, an increase in the total peripheral leucocyte count in guinea pigs after intraperitoneal injection of CSF-HU occurred and this was accompanied by an increase in granulocyte and monocyte counts. A comparison of treatment of experimental Legion, pneumophila pneumonia with CSF-HU alone, ceftazidime alone and a combination revealed lower mortality rates in the group receiving combination therapy. These data suggest a possible function for CSF-HU of enhancing antibiotic therapy in Legionnaires' disease.
研究了人尿集落刺激因子（CSF-HU）对豚鼠肺泡巨噬细胞体外吞噬嗜肺军团菌、产生超氧阴离子以及对嗜肺军团菌的细胞内杀伤作用的影响。肺泡巨噬细胞与CSF-HU一起孵育时，表现出增强的吞噬活性和超氧产生。当处理后的细胞随后与低浓度细菌孵育时，其杀菌活性增强，但当它们以1:10的比例与细菌孵育时，这些细胞未能抑制细菌繁殖。在体内，腹腔注射CSF-HU后豚鼠外周血白细胞总数增加，同时粒细胞和单核细胞计数也增加。对单独使用CSF-HU、单独使用头孢他啶以及联合治疗实验性嗜肺军团菌肺炎的疗效比较显示，接受联合治疗的组死亡率较低。这些数据表明CSF-HU在军团病中增强抗生素治疗可能具有一定作用。
