Kudo Fumitaka, Eguchi Tadashi
Department of Chemistry, Tokyo Institute of Technology, Meguro-ku, Tokyo, Japan.
J Antibiot (Tokyo). 2009 Sep;62(9):471-81. doi: 10.1038/ja.2009.76. Epub 2009 Jul 31.
Biosynthetic studies of aminoglycoside antibiotics have progressed remarkably during the last decade. Many biosynthetic gene clusters for aminoglycoside antibiotics including streptomycin, kanamycin, butirosin, neomycin and gentamicin have been identified to date. In addition, most butirosin and neomycin biosynthetic enzymes have been functionally characterized using recombinant proteins. Herein, we reanalyze biosynthetic genes for structurally related 2-deoxystreptamine (2DOS)-containing aminoglycosides, such as kanamycin, gentamicin and istamycin, based on genetic information including characterized biosynthetic enzymes in neomycin and butirosin biosynthetic pathways. These proposed enzymatic functions for uncharacterized enzymes are expected to support investigation of the complex biosynthetic pathways for this important class of antibiotics.
在过去十年中，氨基糖苷类抗生素的生物合成研究取得了显著进展。迄今为止，已经鉴定出许多氨基糖苷类抗生素的生物合成基因簇，包括链霉素、卡那霉素、丁胺卡那霉素、新霉素和庆大霉素。此外，大多数丁胺卡那霉素和新霉素生物合成酶已通过重组蛋白进行了功能表征。在此，我们基于包括新霉素和丁胺卡那霉素生物合成途径中已表征的生物合成酶在内的遗传信息，重新分析了与结构相关的含2-脱氧链霉胺（2DOS）的氨基糖苷类抗生素（如卡那霉素、庆大霉素和异他霉素）的生物合成基因。这些针对未表征酶提出的酶功能有望支持对这类重要抗生素复杂生物合成途径的研究。
