替勃龙抑制卵巢切除并喂食胆固醇的兔子的主动脉粥样硬化病变形成。

Tibolone inhibits aortic atherosclerotic lesionformation in oophorectomized cholesterol-fed rabbits.

作者信息

Castelo-Branco Camil, Sanjuán Alex, Ascaso Carles, Colodrón Marta, Blümel Juan Enrique, Casals Elena, Ordi Jaume, Vanrell Juan Antonio

机构信息

Menopause Clinic, Department of Gynecology and Obstetrics, Hospital Clínic Provincial, University of Barcelona, Barcelona, Spain;

出版信息

Exp Clin Cardiol. 2003 Spring;8(1):21-5.

PMID:19644583

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2716195/

Abstract

BACKGROUND

Tibolone is a synthetic steroid effective for the treatment of climacteric symptoms and osteoporosis. Long term treatment with tibolone is associated with a significant decrease in cholesterol levels due to a parallel decrease in high-density lipoprotein. However, the effect of these changes on atherogenesis is not known.

OBJECTIVE

To investigate the effect of tibolone therapy on aorta atherogenesis.

MATERIAL AND METHODS

Thirty-two New Zealand white rabbits were fed cholesterol-rich feed and studied for four months. The rabbits underwent laparotomy and were randomly assigned to four groups. Twenty-four rabbits underwent bilateral ovariectomy; of these, eight received tibolone (group T), eight received estradiol valerate (group E), eight received placebo after sterilization (group C), and eight were sham operated (group S).

RESULTS

After receiving the cholesterol-rich diet, total levels of cholesterol increased in group C from 3.17+/-0.72 mmol/L to 35.36+/-9.01 mmol/L, in group S from 2.88+/-0.9 mmol/L to 28.76+/-9.442 mmol/L, in group E from 1.69+/-0.44 mmol/L to 1.69+/-0.44 mmol/L and in group T from 2.03+/-0.22 mmol/L to 26.33+/-13.45 mmol/L (no significant differences were observed among the groups at the end of the study). At four months, the cholesterol- rich diet caused atherosclerotic lesions in both treated and untreated rabbits, affecting 30.47+/-12.2%, 24.51+/-16.1%, 17.91+/-10.19% and 10.21+/-6.8% of the aortic surface for groups C, S, E and T, respectively (P<0.01 for treated groups).

CONCLUSION

The principal result from this study was that treatment with tibolone in cholesterol-fed ovariectomized rabbits reduces aortic atherosclerotic lesion formation and that this reduction is not related to plasma lipid levels.

摘要

背景

替勃龙是一种合成类固醇，对治疗更年期症状和骨质疏松症有效。长期使用替勃龙治疗会导致胆固醇水平显著下降，因为高密度脂蛋白也会同时下降。然而，这些变化对动脉粥样硬化形成的影响尚不清楚。

目的

研究替勃龙治疗对主动脉粥样硬化形成的影响。

材料与方法

32只新西兰白兔喂食富含胆固醇的饲料，并进行为期四个月的研究。兔子接受剖腹手术，并随机分为四组。24只兔子接受双侧卵巢切除术；其中，8只接受替勃龙治疗（T组），8只接受戊酸雌二醇治疗（E组），8只绝育后接受安慰剂治疗（C组），8只接受假手术（S组）。

结果

接受富含胆固醇的饮食后，C组胆固醇总水平从3.17±0.72 mmol/L升至35.36±9.01 mmol/L，S组从2.88±0.9 mmol/L升至28.76±9.442 mmol/L，E组从1.69±0.44 mmol/L升至1.69±0.44 mmol/L，T组从2.03±0.22 mmol/L升至26.33±13.45 mmol/L（研究结束时各组之间未观察到显著差异）。四个月时，富含胆固醇的饮食在治疗和未治疗的兔子中均引起动脉粥样硬化病变，C组、S组、E组和T组分别影响主动脉表面的30.47±12.2%、24.51±16.1%、17.91±10.19%和10.21±6.8%（治疗组P<0.01）。