National Bureau of Fish Genetic Resources, Canal Ring Road, Dilkusha P.O., Lucknow, 226002, India.
Mol Biol Rep. 2010 Apr;37(4):2043-8. doi: 10.1007/s11033-009-9659-3. Epub 2009 Jul 31.
A new cell culture system (MRH) was developed for the first time from 2 months old freshwater prawn, Macrobrachium rosenbergii. Primary cultures were developed from heart tissues by explant culture technique. Cell outgrowth was obtained from the heart explant after 14 days of explant culture. The culture medium used was Leibovitz-15 supplemented with 20% Fetal Bovine Serum along with 1% prawn hemolymph serum, 0.1% glucose, 0.5% NaCl and antibiotics (Penicillin 10,000 Units ml(-1), Streptomycin 10,000 microg ml(-1), Amphotericin B 500 mg ml(-1)) with a final osmomolality of 470-550 mmol kg(-1). The pH of the growth medium found suitable for the growth of the cells was 7.20. The viability of cells was found to be 60% when revived after a month of storage in liquid nitrogen.
首次从 2 个月大的淡水虾Macrobrachium rosenbergii中开发出一种新的细胞培养系统 (MRH)。原代培养通过组织外植体培养技术从心脏组织中进行。心脏外植体培养 14 天后获得细胞外植体。使用的培养基是补充有 20%胎牛血清的 Leibovitz-15，以及 1%虾血血清、0.1%葡萄糖、0.5%NaCl 和抗生素（青霉素 10,000 单位 ml(-1)、链霉素 10,000 微克 ml(-1)、两性霉素 B 500 mg ml(-1)），最终渗透压为 470-550 mmol kg(-1)。发现细胞生长的合适生长培养基的 pH 值为 7.20。在液氮中储存一个月后复苏时，细胞活力为 60%。
