[半枝莲提取物对二乙基亚硝胺诱导的大鼠肝癌的抑制作用]

[Inhibitory effects of Scutellaria barbate extracts on diethylnitrosamine-induced hepatocarcinoma in rats].

作者信息

Dai Zhi-Jun, Wang Xi-Jing, Ji Zong-Zheng, Liu Xiao-Xu, Kang Hua-Feng, Diao Yan, Ma Xiao-Bin, Liu Lei, Ren Hong-Tao

机构信息

Department of Oncology, Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.

出版信息

Zhong Yao Cai. 2009 Apr;32(4):568-71.

PMID:19645244

Abstract

OBJECTIVE

To investigate the inhibitory effects of Scutellaria barbate extracts on diethylnitrosamine-induced hepatocarcinoma in rats.

METHODS

Hepatocarcinoma model rats were induced by diethylnitrosamine (DEN). Sixty SD male rats were randomly divided into 4 groups: normal control group, hepatocarcinoma model group, ESB of high dose group and ESB of low dose group. All rats were killed in the 18th week, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), alkaline phosphatase (ALP), gamma-glutamyltransferase (gamma-GT) and alpha-L-fucosidase (AFU) in serum were measured by biochemical examinations; Hematoxy and eosin (HE) methods were used to examine the changes of liver pathology.

RESULTS

The levels of ALT, AST, TBIL, ALP, gamma-GT, AFU in hepatocarcinoma model group and ESB groups were higher than that of control group (P < 0.05). ESB could relieve hepatic injures. The levels of liver function indexes in ESB groups were lower than that of model group. Histological examination demonstrated that the number of liver cancer nodus in ESB groups were lower than that of model group. Furthermore, ESB could attenuate the grade of cancer cell differentiation.

CONCLUSION

ESB could inhibit experimental hepatocarcinoma and relieve hepatic injures in rats.

摘要

目的

探讨半枝莲提取物对二乙基亚硝胺诱导的大鼠肝癌的抑制作用。

方法

用二乙基亚硝胺（DEN）诱导建立肝癌模型大鼠。将60只SD雄性大鼠随机分为4组：正常对照组、肝癌模型组、高剂量半枝莲提取物组和低剂量半枝莲提取物组。在第18周处死所有大鼠，通过生化检测测定血清中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总胆红素（TBIL）、碱性磷酸酶（ALP）、γ-谷氨酰转移酶（γ-GT）和α-L-岩藻糖苷酶（AFU）的水平；采用苏木精和伊红（HE）染色法检查肝脏病理变化。