Sato-Akaba Hideo, Kuwahara Yoko, Fujii Hirotada, Hirata Hiroshi
Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology, Hokkaido University, Sapporo 060-0814, Japan.
Anal Chem. 2009 Sep 1;81(17):7501-6. doi: 10.1021/ac901169g.
This technical note reports a continuous-wave electron paramagnetic resonance (CW-EPR) imager that can visualize the distribution of free radicals with a half-life of subminutes in three-dimensional (3D) space. A total of 46 EPR spectra under magnetic field gradients, called projections, were obtained for image reconstruction at an interval of 3.6 s. A shortened data-acquisition time was achieved with the use of analog signals that drove field gradient coils in the imager. 3D mapping of the half-lives of nitroxyl radicals (4-hydroxyl-2,2,6,6-tetramethyl-piperidinyl-1-oxyl) was demonstrated in their reduction reaction with ascorbic acid. Inhomogeneous half-lives were clearly mapped pixel-by-pixel in a sample tube.
本技术报告介绍了一种连续波电子顺磁共振（CW-EPR）成像仪，它能够在三维（3D）空间中可视化半衰期为亚分钟级的自由基分布。在磁场梯度下共获得了46个EPR谱（称为投影），以3.6秒的间隔进行图像重建。通过使用驱动成像仪中场梯度线圈的模拟信号，实现了缩短数据采集时间。在硝酰自由基（4-羟基-2,2,6,6-四甲基哌啶-1-氧基）与抗坏血酸的还原反应中，展示了其半衰期的3D映射。在样品管中逐像素清晰地绘制出了不均匀的半衰期。
