Akimov M G, Nazimov I V, Gretskaya N M, Zinchenko G N, Bezuglov V V
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
Biochemistry (Mosc). 2009 Jun;74(6):681-5. doi: 10.1134/s0006297909060133.
Sulfation of N-acyl dopamines has been shown for the first time in cytosolic fractions of rat liver and nervous system. Sulfation of dopamine amides of docosahexaenoic and oleic acids occurred in all tissues studied, N-arachidonoyl dopamine was sulfated in the liver and spinal cord, and N-stearoyl dopamine was sulfated only in the liver. Depending on the substrate and tissue, the sulfation activity varied from 0.5 to 3.5 nmol/min per mg total protein. Kinetic parameters of N-docosahexaenoyl dopamine sulfation in the brain were determined. The findings characterize the sulfation system as the most productive metabolic pathway of N-acyl dopamines, but the role of this system in the body is unclear because of high K(m) value.
N-酰基多巴胺的硫酸化首次在大鼠肝脏和神经系统的胞质部分被发现。二十二碳六烯酸和油酸的多巴胺酰胺在所有研究的组织中均发生硫酸化，N-花生四烯酰多巴胺在肝脏和脊髓中被硫酸化，而N-硬脂酰多巴胺仅在肝脏中被硫酸化。根据底物和组织的不同，硫酸化活性为每毫克总蛋白0.5至3.5 nmol/分钟。测定了大脑中N-二十二碳六烯酰多巴胺硫酸化的动力学参数。这些发现表明硫酸化系统是N-酰基多巴胺最有效的代谢途径，但由于高K(m)值，该系统在体内的作用尚不清楚。
