Szabó I, Maródi L, Karmazsin L, Lakatos L
Department of Pediatrics, University Medical School of Debrecen, Hungary.
Acta Paediatr Hung. 1990;30(3-4):449-59.
Influence of D-Penicillamine (DPA) on metabolic and functional activities of neutrophil granulocytes was investigated in vitro by measuring superoxide anion production and beta-glucuronidase release as well as by determining phagocytic and intracellular killing activities of cells. Preincubation with DPA in the concentration range of 0.5-5.0 mM resulted in 28-53% increase in superoxide anion production by granulocytes stimulated with 10(-7) M FMLP. DPA in the same concentration range resulted in 145-371% rise in the FMLP-stimulated beta-glucuronidase release. However, uptake and subsequent killing of viable Staphylococcus aureus was not influenced by incubation of granulocytes with various concentrations of DPA (0.5-5.0 mM). From these results we conclude that DPA may influence the superoxide anion production and beta-glucuronidase release in granulocytes without altering the phagocytic and intracellular killing activities of these cells. We suppose that the unchanged antibacterial activity of neutrophil granulocytes is resulted by the two opposite DPA effects: acting extracellularly reduces free radical level while the direct membrane effect results in enhancing metabolic activity in granulocytes.
通过测量超氧阴离子产生量、β-葡萄糖醛酸酶释放量以及测定细胞的吞噬和细胞内杀伤活性，在体外研究了D-青霉胺（DPA）对中性粒细胞代谢和功能活性的影响。用浓度范围为0.5 - 5.0 mM的DPA预孵育，可使受10(-7) M 甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）刺激的粒细胞超氧阴离子产生量增加28% - 53%。相同浓度范围的DPA可使FMLP刺激的β-葡萄糖醛酸酶释放量增加145% - 371%。然而，不同浓度（0.5 - 5.0 mM）的DPA孵育粒细胞，对活金黄色葡萄球菌的摄取及随后的杀伤并无影响。从这些结果我们得出结论，DPA可能影响粒细胞中超氧阴离子的产生和β-葡萄糖醛酸酶的释放，而不改变这些细胞的吞噬和细胞内杀伤活性。我们推测中性粒细胞抗菌活性未改变是由DPA的两种相反作用导致的：细胞外作用降低自由基水平，而直接的膜效应导致粒细胞代谢活性增强。
