Korzekwa A, Lukasik K, Skarzynski D J
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Reprod Domest Anim. 2010 Dec;45(6):1089-97. doi: 10.1111/j.1439-0531.2009.01500.x.
The aim of this study was to determine leukotrienes (LTs) functions in the bovine corpus luteum (BCL) during the oestrous cycle. In steroidogenic CL cells we examined the effect of luteotropic [LH, prostaglandin E(2) (PGE(2))] and luteolytic (PGF(2α), cytokines) factors on: the levels of LTB(4) and C(4), the expression of 5-lipoxygenase (LO), LT receptors type I (LTR-I) and LTR-II, and the effects of LTB(4) and C(4) stimulations on the levels of progesterone (P4), PGE(2), F(2α) and nitric oxide (NO) metabolites. Both luteolytic and luteotropic factors stimulated 5-LO expression on days 2-4 and 17-19 of the cycle. Leukotriene receptors type I expression increased after PGE(2) and tumour necrosis factor α with interferon γ (TNF/IFN) stimulation on days 2-4 of the cycle. Leukotriene receptor type II expression increased after PGE(2α) and TNF/IFN stimulation on days 2-4 and 17-19 of the cycle, and LTR-II expression on days 8-10 of the cycle was unchanged after cell stimulation with any factor. Leukotriene B(4) level increased after BSC incubation with luteotropic factors during all examined days of the cycle and after cytokine stimulation at early- and mid-luteal stages, whereas luteolytic factors stimulated LTC(4) secretion over the entire cycle. Leukotriene B(4) stimulated P4 secretion at the mid-luteal stage and stimulated NO secretion during all examined phases. Leukotriene B(4) stimulated PGE(2) secretion at the early- and mid-luteal stage. Leukotriene C(4) inhibited P4 secretion at the mid- and regressing-luteal stage, stimulated NO (entire cycle) and PGF(2α) at mid- and regressing-luteal phases. Leukotrienes modulate steroidogenic cells functions, depending on the stage of the cycle. Leukotriene B(4) plays a luteotropic role stimulating P4 and PGE(2) secretions; LTC(4) stimulates the secretion of luteolytic factors and enhances the luteolytic cascade within BCL.
本研究的目的是确定发情周期中白三烯（LTs）在牛黄体（BCL）中的作用。在类固醇生成的黄体细胞中，我们研究了促黄体生成素[LH、前列腺素E(2)（PGE(2)）]和溶黄体（PGF(2α)、细胞因子）因子对以下方面的影响：白三烯B(4)和C(4)的水平、5-脂氧合酶（LO）的表达、I型白三烯受体（LTR-I）和II型白三烯受体（LTR-II）的表达，以及白三烯B(4)和C(4)刺激对孕酮（P4）、PGE(2)、F(2α)和一氧化氮（NO）代谢产物水平的影响。在发情周期的第2 - 4天和第17 - 19天，溶黄体因子和促黄体生成素因子均刺激5-LO的表达。在发情周期的第2 - 4天，PGE(2)、肿瘤坏死因子α与干扰素γ（TNF/IFN）刺激后，I型白三烯受体表达增加。在发情周期的第2 - 4天和第17 - 19天，PGE(2α)、TNF/IFN刺激后，II型白三烯受体表达增加，且在发情周期的第8 - 10天，任何因子刺激细胞后，LTR-II的表达均无变化。在发情周期的所有检测日，与促黄体生成素因子孵育BSC后，以及在黄体早期和中期细胞因子刺激后，白三烯B(4)水平升高，而溶黄体因子在整个发情周期刺激LTC(4)分泌。白三烯B(4)在黄体中期刺激P4分泌，在所有检测阶段刺激NO分泌。白三烯B(4)在黄体早期和中期刺激PGE(2)分泌。白三烯C(4)在黄体中期和消退期抑制P4分泌，在黄体中期和消退期刺激NO（整个周期）和PGF(2α)分泌。白三烯根据发情周期阶段调节类固醇生成细胞的功能。白三烯B(4)发挥促黄体生成作用，刺激P4和PGE(2)分泌；白三烯C(4)刺激溶黄体因子分泌，并增强牛黄体中的溶黄体级联反应。
