Hashimoto K, Uchikawa R, Tegoshi T, Takeda K, Yamada M, Arizono N
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
Parasite Immunol. 2009 Aug;31(8):457-65. doi: 10.1111/j.1365-3024.2009.01123.x.
To determine the role of T cells and mast cells in intestinal pathology and immune expulsion of intestinal nematodes, worm burdens, goblet cell responses and villus structures were analysed in T- and B-cell-deficient severe combined immunodeficiency (SCID) mice, athymic nu/nu mice and mast cell deficient W/W(v) mice after infection with the nematode Heligmosomoides polygyrus. SCID and nu/nu mice showed significantly higher worm burdens at week 9 post-infection compared with the wild-type controls. SCID and nu/nu mice showed compromised goblet cell hyperplasia and/or Muc 2 expression, indicating that both events are T-cell dependant. On the other hand, the SCID mice showed increased pathology (villus atrophy and crypt hyperplasia) and increased numbers of proliferating cell nuclear antigen positive cells compared to the wild-type controls. W/W(v) mice, conversely, were able to expel the worms normally, had normal goblet cell hyperplasia, and did not demonstrate the changes in mucosal architecture seen in SCID mice, confirming that a normal mast cell response is not necessarily required for these changes. These results suggest that a functional T-cell response, but not a mast cell response, is necessary for anti-parasite responses, goblet cell function, and maintaining normal mucosal architecture.
为了确定T细胞和肥大细胞在肠道病理学以及肠道线虫免疫驱逐中的作用，在感染线虫多房棘球绦虫后，对T细胞和B细胞缺陷的严重联合免疫缺陷（SCID）小鼠、无胸腺裸鼠（nu/nu）和肥大细胞缺陷的W/W（v）小鼠的虫负荷、杯状细胞反应和绒毛结构进行了分析。与野生型对照相比，SCID和nu/nu小鼠在感染后第9周显示出明显更高的虫负荷。SCID和nu/nu小鼠的杯状细胞增生和/或Muc 2表达受损，表明这两个事件均依赖于T细胞。另一方面，与野生型对照相比，SCID小鼠显示出病理学变化增加（绒毛萎缩和隐窝增生）以及增殖细胞核抗原阳性细胞数量增加。相反，W/W（v）小鼠能够正常驱除蠕虫，杯状细胞增生正常，并且没有表现出SCID小鼠中所见的粘膜结构变化，证实这些变化不一定需要正常的肥大细胞反应。这些结果表明，功能性T细胞反应而非肥大细胞反应对于抗寄生虫反应、杯状细胞功能以及维持正常粘膜结构是必要的。
