Morimoto M, Yamauchi Y, Shinkawa S, Ishiwata K, Hayashi T
Laboratory of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
Scand J Immunol. 2006 May;63(5):365-70. doi: 10.1111/j.1365-3083.2006.01754.x.
Mice homologous for the alymphoplasia mutation (aly) show the systemic absence of secondary lymphoid tissues, with disorganized splenic architecture, including the absence of the germinal centre and follicular dendritic cells. In this study, we examined the influence of defects of gut-associated lymphoid tissue (GALT), such as Peyer's patches and the mesenteric lymph nodes, on the host response to helminth infection in aly/aly mice. The present study showed that most of the worms were expelled by day 7 after Nippostrongylus brasiliensis infection in both control aly/+ and aly/aly mice. In aly/aly mice, the number of peripheral blood eosinophils, intestinal goblet cells and mucosal mast cells were increased by N. brasiliensis infection in aly/aly mice to the same level as in the controls. Conversely, aly/aly mice developed more severe Heligmosomoides polygyrus infections than control aly/+ mice, as demonstrated by increased faecal egg counts, with reduced immune responses such as the numbers of intestinal goblet cells and mucosal mast cells. These results suggested that the dependency of GALT in activation of Th2 responses against gastrointestinal nematodes was different depending on the species of nematode.
同源携带无淋巴细胞形成突变（aly）的小鼠表现出全身继发性淋巴组织缺失，脾脏结构紊乱，包括生发中心和滤泡树突状细胞缺失。在本研究中，我们检测了肠道相关淋巴组织（GALT）缺陷，如派尔集合淋巴结和肠系膜淋巴结，对aly/aly小鼠宿主抗蠕虫感染反应的影响。本研究表明，在感染巴西日圆线虫后第7天，大多数蠕虫在对照aly/+小鼠和aly/aly小鼠中均被排出。在aly/aly小鼠中，巴西日圆线虫感染使aly/aly小鼠外周血嗜酸性粒细胞、肠道杯状细胞和黏膜肥大细胞数量增加至与对照相同的水平。相反，aly/aly小鼠比对照aly/+小鼠发生更严重的多枝单睾线虫感染，表现为粪便虫卵计数增加，同时肠道杯状细胞和黏膜肥大细胞数量等免疫反应降低。这些结果表明，GALT在激活针对胃肠道线虫的Th2反应中的依赖性因线虫种类而异。
