Early Merideth A, Lishnevsky Marta, Gilchrist John M, Higgins David M, Orme Ian M, Muller William A, Gonzalez-Juarerro Mercedes, Schenkel Alan R
Department of Microbiology, Immunology & Pathology, Colorado State University, 1682 Campus Delivery Fort Collins, CO 80523-1682, USA.
Exp Mol Pathol. 2009 Oct;87(2):152-8. doi: 10.1016/j.yexmp.2009.07.008. Epub 2009 Jul 28.
Pulse oximetry is a common tool for detecting reduced pulmonary function in human interstitial lung diseases. It has not previously been used in a mouse model of interstitial lung disease. Further, platelet endothelial cell adhesion molecule deficient mice rarely show symptoms until disease is advanced. Using blood oxygen saturation, different stages of disease could be identified in a non-invasive manner. These stages could be correlated to pathology. Collagen deposition, using Picrosirius Red, did correlate with blood oxygen saturation. These studies are the first to show the use of an infrared pulse oximetry system to analyze the progression of a fibrotic interstitial lung disease in a mouse model of the human diseases. Further, these studies show that an early alveolar damage/enlargement event precedes the fibrosis in this mouse model, a stage that represents the best targets for disease analysis and prevention. This stage does not have extensive collagen deposition. Most importantly, targeting this earliest stage of disease for therapeutic intervention may lead to novel treatment for human disease.
脉搏血氧测定法是检测人类间质性肺疾病中肺功能降低的常用工具。此前它尚未用于间质性肺疾病的小鼠模型。此外，血小板内皮细胞黏附分子缺陷小鼠在疾病进展之前很少出现症状。利用血氧饱和度，可以以非侵入性方式识别疾病的不同阶段。这些阶段可以与病理学相关联。使用天狼星红染色检测的胶原蛋白沉积确实与血氧饱和度相关。这些研究首次表明使用红外脉搏血氧测定系统来分析人类疾病小鼠模型中纤维化间质性肺疾病的进展。此外，这些研究表明在该小鼠模型中，早期肺泡损伤/扩大事件先于纤维化，这一阶段是疾病分析和预防的最佳靶点。此阶段没有广泛的胶原蛋白沉积。最重要的是，针对疾病的这个最早阶段进行治疗干预可能会带来人类疾病的新疗法。
