Kubán Petr, Karlberg Bo
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelská 1, CZ-613 00 Brno, Czech Republic.
Anal Chim Acta. 2009 Aug 26;648(2):129-45. doi: 10.1016/j.aca.2009.06.034. Epub 2009 Jun 21.
Various flow sample handling approaches coupled to capillary electrophoresis (CE) are reviewed, covering the research in this field in the 12 years since the milestone year of 1997, when practical interfaces to on-line couple flow injection (FI) and capillary electrophoresis were first developed independently by two research groups. Some previous attempts are also presented. Since 1997 a plethora of ingenious coupled systems have been developed. Although several reviews are available on various aspects of the topic, we have opted for a comprehensive overview of all FI-CE systems, as well as related and similar systems. This coupling has thus also led to the development of systems based on hybrids between the classical and microchip approaches. Truly microchip FI-CE systems are also included in this review. The developed systems have been used for various sample treatments, including on-line membrane-assisted sample treatment, column-based preconcentration, on-line derivatization and monitoring, to name just a few. The utility of coupling flow sampling to CE has been demonstrated in various practical applications that are discussed in detail. The current state-of-the-art and foreseeable future developments are also discussed.
本文综述了与毛细管电泳（CE）联用的各种流动样品处理方法，涵盖了自1997年这一里程碑式年份以来12年间该领域的研究进展。1997年，两个研究小组首次独立开发出了将流动注射（FI）与毛细管电泳在线联用的实用接口。文中还介绍了一些先前的尝试。自1997年以来，已经开发出了大量巧妙的联用系统。尽管已有几篇关于该主题各个方面的综述，但我们还是选择对所有FI-CE系统以及相关和类似系统进行全面概述。这种联用也因此促成了基于经典方法与微芯片方法相结合的系统的发展。本综述还包括真正的微芯片FI-CE系统。所开发的系统已用于各种样品处理，包括在线膜辅助样品处理、基于柱的预富集、在线衍生化和监测等等。流动采样与CE联用的实用性已在各种实际应用中得到证明，本文将对此进行详细讨论。文中还讨论了当前的技术水平以及可预见的未来发展。
