Colyer C L, Tang T, Chiem N, Harrison D J
Department of Chemistry, University of Alberta, Edmonton, Canada.
Electrophoresis. 1997 Sep;18(10):1733-41. doi: 10.1002/elps.1150181006.
Clinical interest in the use of capillary electrophoresis (CE) has recently been extended to the microchip environment. Clinical analyses demand careful handling of complex samples that are often limited in quantity and in concentration. The integrated sample handling and analysis capabilities of microchip substrates thus seem ideally suited to clinical applications. This review surveys the development of sample handling (injection, mixing, and reaction) and separation elements on-chip. The integration of these elements to create a variety of clinical analyzers has been demonstrated. The application of microchip CE systems to human serum protein analysis, immunoassay, and DNA studies is reviewed, along with various other clinical applications. In addition, the clinical potential of the lab-on-a-chip concept is discussed.
最近，毛细管电泳（CE）在临床应用方面的兴趣已扩展到微芯片环境。临床分析需要仔细处理复杂的样本，这些样本的数量和浓度往往有限。因此，微芯片基板的集成样本处理和分析能力似乎非常适合临床应用。本文综述了芯片上样本处理（进样、混合和反应）和分离元件的发展。已经证明了将这些元件集成以创建各种临床分析仪。本文回顾了微芯片CE系统在人血清蛋白分析、免疫测定和DNA研究以及各种其他临床应用中的应用。此外，还讨论了芯片实验室概念的临床潜力。
