Pta1、Pcf11和KlCYC1富含AU元件在酵母RNA 3'端加工选择中的作用。

Involvement of Pta1, Pcf11 and a KlCYC1 AU-rich element in alternative RNA 3'-end processing selection in yeast.

作者信息

Seoane Silvia, Lamas-Maceiras Mónica, Rodríguez-Torres Ana M, Freire-Picos María A

机构信息

Universidade da Coruña, Facultad de Ciencias, Campus da Zapateira S/N, 15071 A Coruña, Spain.

出版信息

FEBS Lett. 2009 Sep 3;583(17):2843-8. doi: 10.1016/j.febslet.2009.07.042. Epub 2009 Jul 30.

DOI:10.1016/j.febslet.2009.07.042

PMID:19646984

Abstract

This work reports the involvement of yeast RNA processing factors Pta1 and Pcf11 in alternative 3'-end RNA processing. The pta1-1 and pcf11-2 mutations changed the predominance of KlCYC1 1.14 and 1.5 kb transcript isoforms. Mutation of the KlCYC1 3'-UTR AU-rich sequence at positions 679-690 (mutant M1) altered transcript predominance. Moreover, expression of M1 in the yeast mutants partially suppressed their effects in the predominance pattern. The combination of the M1 and M2 (694-698 deletion) mutations abolished the alternative processing. Pta1 involvement in this selection was confirmed using the Pta1-td degron strain.

摘要

这项工作报道了酵母RNA加工因子Pta1和Pcf11参与3'端RNA的可变加工。pta1-1和pcf11-2突变改变了KlCYC1 1.14 kb和1.5 kb转录本亚型的优势地位。KlCYC1 3'-UTR中679 - 690位富含AU的序列发生突变（突变体M1）改变了转录本的优势地位。此外，M1在酵母突变体中的表达部分抑制了它们在优势模式中的作用。M1和M2（694 - 698缺失）突变的组合消除了可变加工。使用Pta1-td降解子菌株证实了Pta1参与了这种选择。