Ahyayauch Hasna, Collado M Isabel, Goñi Félix M, Lichtenberg Dov
Unidad de Biofísica (Centro Mixto CSIC-UPV/EHU), Departamento de Bioquímica, Universidad del País Vasco, Bilbao, Spain.
FEBS Lett. 2009 Sep 3;583(17):2859-64. doi: 10.1016/j.febslet.2009.07.046. Epub 2009 Aug 4.
The distribution of phosphatidylcholine (PC) and sphingomyelin (SM) between the solubilized (micellar) and non-solubilized (lamellar) fractions arising from bilayers composed of PC and SM, with or without cholesterol (Chol) has been measured under conditions of partial, incomplete solubilization by Triton X-100. Quantitation is achieved by (31)P-NMR determination of the composition of mixed micelles in the range of bilayer-micelle coexistence. We find that the solubilized fraction of bilayers consisting of binary mixtures of PC and SM is rich in SM, as expected from previous data on solubilization of pure PC and pure SM liposomes. In contrast, after partial solubilization of ternary mixtures of PC, SM and Chol, the solubilized fraction becomes SM-poor, as observed in the partial solubilization of biomembranes.
在由Triton X-100进行部分、不完全溶解的条件下，已测量了由磷脂酰胆碱（PC）和鞘磷脂（SM）组成的双层膜（有或没有胆固醇（Chol））产生的溶解（胶束）部分和未溶解（层状）部分之间PC和SM的分布。通过（31）P-NMR测定双层-胶束共存范围内混合胶束的组成来实现定量。我们发现，由PC和SM二元混合物组成的双层膜的溶解部分富含SM，这与先前关于纯PC和纯SM脂质体溶解的数据预期一致。相比之下，在PC、SM和Chol三元混合物部分溶解后，溶解部分变得贫SM，这与生物膜的部分溶解情况一致。
