血管内皮细胞中响应球状脂联素的基因表达诱导
Induction of gene expression in response to globular adiponectin in vascular endothelial cells.
作者信息
Tomizawa Atsuko, Hattori Yoshiyuki, Kasai Kikuo
机构信息
Department of Endocrinology and Metabolism, Dokkyo University School of Medicine, Mibu, Tochigi, Japan.
出版信息
Life Sci. 2009 Sep 9;85(11-12):457-61. doi: 10.1016/j.lfs.2009.07.012. Epub 2009 Aug 6.
AIMS
Adiponectin is an adipocyte-specific protein that plays an important regulatory role in the development or prevention of diabetes and atherosclerosis.
MAIN METHODS
In the present study, we examined the effect of a proteolytic cleavage product of adiponectin, known as globular adiponectin (gAd), on induction of gene expression and activation of various signaling pathways in vascular endothelial cells.
KEY FINDINGS
We showed that gAd induces the expression of a number of genes using PCR arrays, including MCP-1, VCAM-1, E-selectin, IL-6, and IL-8, all of which have been previously shown to be associated with adiponectin, as well as SOD2, PAI-1, and CSF2, which is a new finding. We also demonstrated that gAd activates AMPK, Akt, and NF-kB, as well as various MAPKs, including ERK1/2, JNK, and p38MAPK.
SIGNIFICANCE
Upstream regulation of gene expression might involve two or more activated pathways which interact with one another.
目的
脂联素是一种脂肪细胞特异性蛋白，在糖尿病和动脉粥样硬化的发生或预防中发挥重要的调节作用。
主要方法
在本研究中，我们检测了脂联素的一种蛋白水解裂解产物，即球状脂联素（gAd），对血管内皮细胞中基因表达的诱导作用以及各种信号通路的激活情况。
主要发现
我们通过PCR芯片显示gAd可诱导多种基因的表达，包括MCP-1、VCAM-1、E-选择素、IL-6和IL-8，所有这些基因先前已被证明与脂联素有关，以及SOD2、PAI-1和CSF2，这是一个新发现。我们还证明gAd可激活AMPK、Akt和NF-κB，以及各种丝裂原活化蛋白激酶（MAPK），包括ERK1/2、JNK和p38MAPK。
意义
基因表达的上游调节可能涉及两个或更多相互作用的激活通路。
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