Detection of various types of human papillomaviruses in premalignant and malignant cervical lesions using DNA-DNA in situ hybridization.

Detection of human papillomavirus (HPV) in formalin-fixed paraffin embedded tissue sections by in situ hybridization technique employing biotinylated (HPV) DNA probe 6/11, 16/18 and 31/33/35 was done retrospectively in 25 cases of cervical dysplasia, 32 cases of cervical squamous cell carcinoma in situ, 52 cases of invasive cervical squamous cell carcinoma and 7 cases of adenocarcinoma. HPV could be demonstrated in 10 cases (40.00%) of cervical dysplasia, 8 cases (25.00%) of cervical squamous cell carcinoma in situ and 31 cases (59.61%) of invasive cervical squamous cell carcinoma and 4 cases (57.14%) of adenocarcinoma. Among the dysplastic cases, 4 cases showed HPV 31/33/35, 4 cases showed HPV 16/18 together with HPV 31/33/35, 1 case showed mixed typing of HPV 6/11 and 31/33/35, and 1 case showed mixed typing of HPV 6/11, 16/18 and 31/33/35. In 32 cases of squamous cell carcinoma in situ, 1 case of HPV 6/11; 3 cases of HPV 16/18, 3 cases of HPV 31/33/35 and 1 case of mixed typing of HPV 16/18 and 31/33/35 were present. A case of HPV 6/11, 10 cases of HPV 16/18 and 9 cases of HPV 31/33/35 could be detected among the cases of invasive squamous cell carcinoma. Mixed typing of HPV 6/11 and 16/18; HPV 6/11 and 31/33/35; HPV 16/18 and 31/33/35; HPV 6/11, 16/18 and 31/33/35 were revealed in 2, 1, 3 and 5 cases of invasive squamous cell carcinoma, respectively. In 7 cases of adenocarcinoma, 1, 2 and 1 cases exhibited positivity for HPV 16/18; HPV 6/11 and HPV 16/18; and HPV 16/18 and HPV 31/33/35.(ABSTRACT TRUNCATED AT 250 WORDS)