Desjardins G C, Brawer J R, Beaudet A
Department of Anatomy, McGill University, Montreal, Que., Canada.
Brain Res. 1990 Dec 17;536(1-2):114-23. doi: 10.1016/0006-8993(90)90015-4.
The radioautographic distribution of mu, delta and kappa opioid binding sites was examined by in vitro radioautography in the rat hypothalamus using the highly selective ligands [125I]-FK 33-824, [125I]azidoDTLET and [125I]DPDYN, respectively. Levels of mu opioid binding sites varied considerably amongst hypothalamic nuclei. mu Opioid labeling was dense in the medial preoptic area, medial preoptic nucleus, suprachiasmatic nucleus and ventromedial nucleus, whilst the supraoptic nucleus, paraventricular nucleus, arcuate nucleus and dorsomedial nucleus were devoid of labeling. Delta opioid labeling was sparse throughout most of the hypothalamus; however, moderate binding densities were detected in the suprachiasmatic and ventromedial nucleus. kappa Opioid labeling was also scant throughout the hypothalamus with the exception of the suprachiasmatic nucleus which was very densely labeled. Our results indicate that the 3 opioid receptors types are differentially distributed within the hypothalamus, although a significant overlap exists. In general, the distribution of hypothalamic opioid receptors correlates well with that of opioid-containing terminal fibers and may represent the anatomical substrate for opioid involvement in the hypothalamic regulation of autonomic, behavioral and neuroendocrine functions.
分别使用高选择性配体[125I]-FK 33-824、[125I]叠氮基DTLET和[125I]DPDYN，通过体外放射自显影术在大鼠下丘脑检测μ、δ和κ阿片样物质结合位点的放射自显影分布。μ阿片样物质结合位点的水平在下丘脑核团中差异很大。μ阿片样物质标记在内侧视前区、内侧视前核、视交叉上核和腹内侧核中密集，而视上核、室旁核、弓状核和背内侧核没有标记。δ阿片样物质标记在大部分下丘脑稀疏；然而，在视交叉上核和腹内侧核中检测到中等结合密度。κ阿片样物质标记在下丘脑也很少，除了视交叉上核被非常密集地标记。我们的结果表明，3种阿片样物质受体类型在下丘脑中分布不同，尽管存在显著重叠。一般来说，下丘脑阿片样物质受体的分布与含阿片样物质的终末纤维的分布很好地相关，并且可能代表阿片样物质参与下丘脑对自主、行为和神经内分泌功能调节的解剖学基础。
