UPMC Univ Paris 6, Laboratoire ARAGO, UMR 7621, Observatoire Océanologique, F-66651 Banyuls/mer, France.
Laboratoire de Microbiologie, de Géochimie et d'Ecologie Marines, CNRS-UMR 6117, Centre d'Océanologie de Marseille, Campus de Luminy, Case 901, 13288 Marseille Cedex 9, France.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):209-213. doi: 10.1099/ijs.0.011460-0. Epub 2009 Jul 31.
A novel Gram-negative bacteria, named CZ41_10a(T), was isolated from coastal surface waters of the north-western Mediterranean Sea. Cells were motile, pleomorphic rods, 1.6 mum long and 0.7 mum wide and formed cream colonies on marine agar medium. The G+C content of the genomic DNA was 65 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the new isolate in the genus Thalassobaculum, a member of the family Rhodospirillaceae, class Alphaproteobacteria. Unlike Thalassobaculum litoreum CL-GR58(T), its closest relative, strain CZ41_10a(T) was unable to grow anaerobically and did not exhibit nitrate reductase activity. On the basis of DNA-DNA hybridization, fatty acid content and physiological and biochemical characteristics, this isolate represents a novel species for which the name Thalassobaculum salexigens sp. nov. is proposed. The type strain is CZ41_10a(T) (=DSM 19539(T)=CIP 109604(T)=MOLA [corrected] 84(T)). An emended description of the genus Thalassobaculum is also given.
从西北地中海的沿海表层水中分离到一株新型革兰氏阴性菌，命名为 CZ41_10a(T)。细胞呈运动、多形杆状，长 1.6 µm，宽 0.7 µm，在海洋琼脂培养基上形成奶油色菌落。基因组 DNA 的 G+C 含量为 65mol%。16S rRNA 基因序列的系统发育分析将新分离株置于海洋杆菌属（Rhodospirillaceae 科，α变形菌纲）中，是 Thalassobaculum 的一个成员。与最接近的亲缘种 Thalassobaculum litoreum CL-GR58(T)不同，菌株 CZ41_10a(T)不能进行厌氧生长，也没有表现出硝酸盐还原酶活性。基于 DNA-DNA 杂交、脂肪酸含量以及生理生化特征，该分离株代表了一个新的物种，建议将其命名为 Salexigens thalassobaculum sp. nov.。模式菌株为 CZ41_10a(T)=DSM 19539(T)=CIP 109604(T)=MOLA [更正] 84(T)。对 Thalassobaculum 属的描述也进行了修正。
