Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, 53127 Bonn, Germany.
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):149-153. doi: 10.1099/ijs.0.012211-0. Epub 2009 Jul 31.
The taxonomic position of an actinomycete, strain IMMIB L-889(T), isolated from the sputum of a 64-year-old man, was determined using a polyphasic taxonomic approach. The strain had chemical and morphological properties that were consistent with its classification in the genus Actinomadura. It formed a distinct phyletic line in the 16S rRNA gene tree of Actinomadura and was most closely related to the type strain of Actinomadura hallensis (98.4 % sequence similarity), but could be readily distinguished from the latter species using DNA-DNA relatedness and phenotypic data. The combined genotypic and phenotypic data indicate that strain IMMIB L-889(T) represents a novel species of the genus Actinomadura, for which the name Actinomadura sputi sp. nov. is proposed. The type strain is IMMIB L-889(T) =DSM 45233(T)=CCUG 56589(T). [corrected]
一株放线菌菌株 IMMIB L-889(T)的分类地位采用多相分类法确定，该菌株从一位 64 岁男性的痰液中分离得到。该菌株的化学和形态特征与其在 Actinomadura 属中的分类一致。它在 Actinomadura 的 16S rRNA 基因树中形成了一个独特的进化枝，与 Actinomadura hallensis 的模式株最为密切相关（98.4%序列相似性），但可以通过 DNA-DNA 同源性和表型数据与后者轻易区分。综合基因型和表型数据表明，菌株 IMMIB L-889(T)代表 Actinomadura 属的一个新种，现提议将其命名为 Actinomadura sputi sp. nov.。模式菌株为 IMMIB L-889(T)=DSM 45233(T)=CCUG 56589(T)。[已更正]
