Abagana Adam Yacoub, Sun Pengyu, Liu Chongxi, Cao Tingting, Zheng Weiwei, Zhao Shanshan, Xiang Wensheng, Wang Xiangjing
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Antonie Van Leeuwenhoek. 2016 Jun;109(6):833-9. doi: 10.1007/s10482-016-0683-8. Epub 2016 Mar 24.
A novel single spore-producing actinomycete, designated strain NEAU-Gz5(T), was isolated from a soil sample from Gama, Chad. A polyphasic taxonomic study was carried out to establish the status of this strain. The diamino acid present in the cell wall is meso-diaminopimelic acid. Glucose, mannose and madurose occur in whole cell hydrolysates. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and an unidentified glycolipid. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The predominant cellular fatty acids were found to be C16:0, iso-C15:0, iso-C16:0 and C18:0 10-methyl. Phylogenetic analysis based on the 16S rRNA gene showed that strain NEAU-Gz5(T) belongs to the genus Actinomadura and is closely related to Actinomadura oligospora JCM 10648(T) (ATCC 43269(T); 98.3 % similarity). However, the low level of DNA-DNA relatedness and some different phenotypic characteristics allowed the strain to be distinguished from its close relatives. Therefore, it is concluded that strain NEAU-Gz5(T) represents a novel species of the genus of Actinomadura, for which the name Actinomadura gamaensis sp. nov. is proposed. The type strain is NEAU-Gz5(T) (= CGMCC 4.7301(T) = DSM 100815(T)).
从乍得加马的一份土壤样品中分离出一株新型的产单孢子放线菌，命名为菌株NEAU-Gz5(T)。开展了多相分类研究以确定该菌株的分类地位。细胞壁中的二氨基酸为内消旋二氨基庚二酸。全细胞水解物中含有葡萄糖、甘露糖和马杜拉糖。发现极性脂类由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇甘露糖苷和一种未鉴定的糖脂组成。主要的甲基萘醌被鉴定为MK-9(H8)和MK-9(H6)。主要的细胞脂肪酸为C16:0、异-C15:0、异-C16:0和C18:0 10-甲基。基于16S rRNA基因的系统发育分析表明，菌株NEAU-Gz5(T)属于马杜拉放线菌属，与少孢马杜拉放线菌JCM 10648(T)（ATCC 43269(T)；相似度98.3%）密切相关。然而，低水平的DNA-DNA相关性和一些不同的表型特征使得该菌株能够与其近缘菌株区分开来。因此，得出结论，菌株NEAU-Gz5(T)代表马杜拉放线菌属的一个新物种，为此提出新名称加马马杜拉放线菌（Actinomadura gamaensis sp. nov.）。模式菌株为NEAU-Gz5(T)（=CGMCC 4.7301(T)=DSM 100815(T)）。
