Ishida M, Akagi H, Shimamoto K, Ohfune Y, Shinozaki H
Tokyo Metropolitan Institute of Medical Science, Japan.
Brain Res. 1990 Dec 24;537(1-2):311-4. doi: 10.1016/0006-8993(90)90375-l.
The (2S,3S,4S) isomer of alpha-(carboxycyclopropyl)glycine (L-CCG-I), a conformationally restricted glutamate analogue, caused a marked depolarization of motoneurons in the isolated rat spinal cord, which was almost insensitive to CPP and CNQX. Depolarizing responses to L-CCG-I were markedly decreased by reducing the temperature of the bathing fluid. Similar results were obtained in the case of trans-ACPD, which is a metabotropic glutamate receptor agonist, but the depolarizing action of L-CCG-I was more potent than that of trans-ACPD. In Xenopus oocytes injected with poly(A)+ mRNA extracted from the rat brain, L-CCG-I induced significant oscillatory chloride currents, suggesting that L-CCG-I is a potent agonist for metabotropic-type glutamate receptors.
α-（羧基环丙基）甘氨酸（L-CCG-I）的（2S，3S，4S）异构体是一种构象受限的谷氨酸类似物，可使离体大鼠脊髓中的运动神经元发生明显去极化，这种去极化对CPP和CNQX几乎不敏感。降低灌流液温度可显著降低对L-CCG-I的去极化反应。代谢型谷氨酸受体激动剂反式-ACPD也得到了类似结果，但L-CCG-I的去极化作用比反式-ACPD更强。在注射了从大鼠脑中提取的聚腺苷酸加尾mRNA的非洲爪蟾卵母细胞中，L-CCG-I诱导出显著的振荡性氯离子电流，这表明L-CCG-I是代谢型谷氨酸受体的有效激动剂。
