Tanabe S, Ito I, Sugiyama H
Department of Biology, Faculty of Science, Okayama University, Japan.
Neurosci Res. 1991 Feb;10(1):71-7. doi: 10.1016/0168-0102(91)90021-p.
Pharmacological properties of metabotropic glutamate receptors were studied in Xenopus oocytes injected with rat brain mRNA. trans-1-Amino-cyclopentyl-1,3-dicarboxylic acid (t-ACPD), a conformationally restricted analog of glutamate, induced oscillatory inward currents in mRNA-injected oocytes. These t-ACPD responses showed several characteristics identical to those of the other metabotropic responses including the metabotropic glutamate responses stimulated by quisqualate. D,L-2-Amino-3-phophonopropionate (D,L-AP3) effectively suppressed the t-ACPD and ibotenate responses. However, quisqualate responses were not affected substantially by D,L-AP3. These findings suggest that the metabotropic glutamate receptors in the oocytes may be classified into at least two subtypes according to their pharmacological properties: one preferentially activated by quisqualate and insensitive to AP3, and the other activated by t-ACPD and ibotenate and antagonized by AP3.
在注射了大鼠脑信使核糖核酸（mRNA）的非洲爪蟾卵母细胞中研究了代谢型谷氨酸受体的药理学特性。反式-1-氨基环戊基-1,3-二羧酸（t-ACPD），一种谷氨酸的构象受限类似物，在注射mRNA的卵母细胞中诱导出振荡性内向电流。这些t-ACPD反应表现出与其他代谢型反应相同的几个特征，包括由喹啉酸刺激的代谢型谷氨酸反应。D，L-2-氨基-3-磷酸丙酸（D，L-AP3）有效地抑制了t-ACPD和鹅膏蕈氨酸反应。然而，喹啉酸反应基本上不受D，L-AP3的影响。这些发现表明，卵母细胞中的代谢型谷氨酸受体根据其药理学特性可能至少可分为两种亚型：一种优先被喹啉酸激活且对AP3不敏感，另一种被t-ACPD和鹅膏蕈氨酸激活并被AP3拮抗。
