Hoogenkamp M A, Crielaard W, ten Cate J M, Wever R, Hartog A F, Renirie R
Department of Cariology, Endodontology, Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands.
Caries Res. 2009;43(5):334-8. doi: 10.1159/000231569. Epub 2009 Aug 1.
The aim of this study was to investigate the antimicrobial activity of vanadium chloroperoxidase (VCPO) reaction products on planktonic and biofilm cells of Streptococcus mutans C180-2. Planktonic and biofilm cells were incubated in a buffered reaction mixture containing VCPO, halide (either chloride or bromide) and hydrogen peroxide, and the killing efficacy was assessed by CFU counts. The enzymatic products formed by VCPO significantly reduced the viability of planktonic and biofilm cells compared to their negative controls and the effect on the biofilm cells was more effective than a 0.2% chlorhexidine digluconate treatment. We conclude that VCPO and its reaction products form a potent antimicrobial system against S. mutans.
本研究的目的是调查钒氯过氧化物酶（VCPO）反应产物对变形链球菌C180-2浮游细胞和生物膜细胞的抗菌活性。将浮游细胞和生物膜细胞在含有VCPO、卤化物（氯化物或溴化物）和过氧化氢的缓冲反应混合物中孵育，并通过菌落形成单位计数评估杀菌效果。与阴性对照相比，VCPO形成的酶产物显著降低了浮游细胞和生物膜细胞的活力，并且对生物膜细胞的作用比0.2%葡萄糖酸氯己定处理更有效。我们得出结论，VCPO及其反应产物形成了一种针对变形链球菌的有效抗菌系统。
