Cobos-Correa Amanda, Trojanek Johanna B, Diemer Stefanie, Mall Marcus A, Schultz Carsten
[1] Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany. [2] Molecular Medicine Partnership Unit, European Molecular Biology Laboratory and University of Heidelberg, Heidelberg, Germany.
Nat Chem Biol. 2009 Sep;5(9):628-30. doi: 10.1038/nchembio.196. Epub 2009 Aug 2.
MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.
基质金属蛋白酶12（MMP12）是一种与炎症相关的金属蛋白酶。为监测其活性，我们基于荧光共振能量转移（FRET）合成了一种膜靶向报告分子（LaRee1）。与现有传感器不同，LaRee1通过FRET的丧失以及脂化片段的内化来检测MMP12活性。在肺部炎症小鼠模型的支气管肺泡灌洗中，LaRee1检测到活化巨噬细胞表面的MMP12活性。LaRee1可能成为监测肺部疾病的有力工具。
