从人或小鼠气道巨噬细胞中分离细胞外囊泡用于功能测定及体内或体外实验的方案。

Protocol for isolating extracellular vesicles from human or mouse airway macrophages for functional assays and in vivo or in vitro experimentation.

作者信息

Sari Ezgi, Richter Robert P, Stacks Delores A, Lewis Crystal, Smith Julian B, Xu Ningyong, Finley Dakota C, Genschmer Kristopher R, Margaroli Camilla

机构信息

University of Alabama at Birmingham, Graduate Biomedical Sciences, Birmingham, AL 35233, USA; Department of Pathology, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, AL 35233, USA.

Department of Pediatrics, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, AL 35233, USA.

出版信息

STAR Protoc. 2025 Jun 20;6(2):103867. doi: 10.1016/j.xpro.2025.103867. Epub 2025 Jun 2.

Macrophages are key players in host defense and lung injury, and their crosstalk with other cells dictates the course of the inflammatory response and tissue remodeling. Here, we present a protocol for isolating extracellular vesicles (EVs) from human bronchoalveolar lavage fluid (BALF) and for generating EVs from primary human or murine alveolar macrophages following stimulation by cigarette smoke extract. Furthermore, we describe how to measure matrix metalloproteinase (MMP)-12 activity on macrophage-derived EVs using a Förster resonance energy transfer assay.