具有表面等离激元驻波的宽场扩展分辨率荧光显微镜术
Wide-field extended-resolution fluorescence microscopy with standing surface-plasmon-resonance waves.
作者信息
Chung Euiheon, Kim Yang-Hyo, Tang Wai Teng, Sheppard Colin J R, So Peter T C
机构信息
Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
出版信息
Opt Lett. 2009 Aug 1;34(15):2366-8. doi: 10.1364/ol.34.002366.
Abstract
The resolution of conventional surface-plasmon-resonance (SPR) imaging has been limited by the diffraction nature of light. A wide-field extended-resolution optical imaging technique, standing-wave SPR fluorescence (SW-SPRF) microscopy, has been developed. Based on evanescent SPR standing waves, SW-SPRF provides lateral resolution approaching 100 nm and offers the advantages of significant signal enhancement and background-noise reduction. SW-SPRF has the potential for sensitive biomolecular detection, nanoscale imaging, and lithographic applications.
摘要
传统表面等离子体共振（SPR）成像的分辨率一直受光的衍射特性限制。一种宽场扩展分辨率光学成像技术——驻波SPR荧光（SW-SPRF）显微镜已被开发出来。基于倏逝SPR驻波，SW-SPRF提供接近100纳米的横向分辨率，并具有显著增强信号和降低背景噪声的优点。SW-SPRF在灵敏的生物分子检测、纳米级成像和光刻应用方面具有潜力。
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