使用SPOT技术和CelluSpots方法合成肽阵列。

Synthesis of peptide arrays using SPOT-technology and the CelluSpots-method.

作者信息

Winkler Dirk F H, Hilpert Kai, Brandt Ole, Hancock Robert E W

机构信息

Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, BC, Canada.

出版信息

Methods Mol Biol. 2009;570:157-74. doi: 10.1007/978-1-60327-394-7_5.

DOI:10.1007/978-1-60327-394-7_5

PMID:19649591

Abstract

Peptide synthesis on cellulose using the SPOT technology follows the standard Fmoc-chemistry and can be performed manually or automated. This method allows the synthesis of low-cost peptide arrays containing around 900 large spots of addressable peptides on a cellulose sheet of 19 cm x 29 cm. These peptides can be cleaved from the cellulose support by ammonia gas and afterward spotted on glass microchips. Alternatively, the peptides can be synthesized on modified cellulose discs and CelluSpot microarrays can be produced.

摘要

使用SPOT技术在纤维素上进行肽合成遵循标准的Fmoc化学方法，可手动或自动进行。该方法能够在一张19厘米×29厘米的纤维素片上合成低成本的肽阵列，其中包含约900个可寻址肽的大斑点。这些肽可以通过氨气从纤维素载体上切割下来，然后点样到玻璃微芯片上。或者，肽也可以在修饰的纤维素圆盘上合成，从而制备CelluSpot微阵列。