Souplet Vianney, Roux Clément, Melnyk Oleg
UMR CNRS 8161 Institut de Biologie de Lille 1, Lille Cedex, France.
Methods Mol Biol. 2009;570:287-97. doi: 10.1007/978-1-60327-394-7_14.
Microarrays are frequently prepared on microscope glass slides. However, glass substrates can break or cut and thus can lead to the contamination of the manipulator during the analysis of biological samples. Alternately, bisphenol A polycarbonate (PC) is shock-resistant and, in addition, is easily eliminated by incineration. We show here that PC is a useful substrate for peptide microarray preparation. We describe in particular the preparation of peptide microarrays on PC using semicarbazide-functionalized silica nanoparticles and in situ semicarbazone ligation with glyoxylyl-peptides. The microarrays were used for the detection of antibodies using fluorescence detection.
微阵列通常制备在显微镜载玻片上。然而，玻璃基质可能会破裂或划伤，因此在生物样品分析过程中可能导致机械手受到污染。另外，双酚A聚碳酸酯（PC）具有抗冲击性，并且此外，通过焚烧很容易消除。我们在此表明，PC是用于制备肽微阵列的有用基质。我们特别描述了使用氨基脲功能化的二氧化硅纳米颗粒在PC上制备肽微阵列以及与乙醛酰基肽进行原位缩氨基脲连接。这些微阵列用于通过荧光检测来检测抗体。
