Köhn Maja
European Molecular Biology Laboratory, Gene Expression Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
J Pept Sci. 2009 Jun;15(6):393-7. doi: 10.1002/psc.1130.
Protein, peptide and small molecule microarrays are valuable tools in biological research. In the last decade, substantial progress has been achieved to make these powerful technologies more reliable and available for researchers. This review describes chemical preparation methods for these microarrays with focus on site-selective and bioorthogonal immobilization reactions, particularly the Staudinger ligation and the thiol-ene reaction. In addition, the application of peptide microarrays, which were prepared by Staudinger ligation, to substrate specificity mapping is illustrated.
蛋白质、肽和小分子微阵列是生物学研究中的宝贵工具。在过去十年中，已取得了重大进展，以使这些强大的技术对研究人员而言更可靠且更易获得。本综述描述了这些微阵列的化学制备方法，重点是位点选择性和生物正交固定反应，特别是施陶丁格连接反应和硫醇-烯反应。此外，还展示了通过施陶丁格连接反应制备的肽微阵列在底物特异性图谱绘制中的应用。
