Ventura J, Gutiérrez-Sanchez G, Rodríguez-Herrera R, Aguilar C N
Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Coahuila, México.
Folia Microbiol (Praha). 2009;54(3):199-203. doi: 10.1007/s12223-009-0031-8. Epub 2009 Aug 2.
'Tar bush' and 'creosote bush' were substrates of fungal cultivation for tannase production and gallic acid and pyrocatechol accumulation. Aspergillus niger GH1 grew similarly on both plant materials under solid state culture conditions, reaching maximal levels after 4 d. Fungal strain degraded all tannin content of creosote bush after 4 d of fermentation and >75 % of tar bush after 5 d. Higher level of tannase activity was detected in tar bush fermentation. Biotransformation of tannins to gallic acid was high (93 % in creosote bush and 89 % in tar bush). Pyrocatechol was released poorly. Kinetic parameters of tannin conversion were calculated.
“焦油灌木”和“石炭酸灌木”是用于生产单宁酶以及积累没食子酸和邻苯二酚的真菌培养底物。在固态培养条件下，黑曲霉GH1在这两种植物材料上的生长情况相似，4天后达到最高水平。发酵4天后，该真菌菌株降解了石炭酸灌木的所有单宁含量，5天后降解了焦油灌木单宁含量的75%以上。在焦油灌木发酵中检测到更高水平的单宁酶活性。单宁向没食子酸的生物转化程度很高（石炭酸灌木中为93%，焦油灌木中为89%）。邻苯二酚的释放量很少。计算了单宁转化的动力学参数。
