Brydak L, Sadowski W
Zakład Wirusologii PZH w Warszawie.
Med Dosw Mikrobiol. 1990;42(1-2):50-3.
Swiss white mice were given intranasally suspension of influenza A virus (H3N2) isolated at different period of time and replicated in lowered temperatures in 11 days old chicken embryos. The presence of antigen in lung of animals was detected by IF. They were given the virus replicated at 30 degrees C at different rate depending on strain tested. No distinct differences were observed in haemagglutination inhibition antibody level. On the other hand the level of neuraminidase activity inhibiting antibodies level was significantly higher after giving virus replicated at 30 degrees C than after giving the virus replicated at 37 degrees C. In the case of epidemic strains 4-5 fold fold increase of immunogenicity of neuraminidase component was observed and in the remaining strains immunogenicity of neuraminidase increased 1-5-fold only.
将在不同时间段分离并在11日龄鸡胚中低温复制的甲型流感病毒（H3N2）鼻内悬液接种给瑞士小白鼠。通过免疫荧光法检测动物肺部抗原的存在。根据所测试的毒株，以不同比例给它们接种在30℃复制的病毒。在血凝抑制抗体水平上未观察到明显差异。另一方面，接种在30℃复制的病毒后，神经氨酸酶活性抑制抗体水平显著高于接种在37℃复制的病毒后。对于流行毒株，观察到神经氨酸酶成分的免疫原性增加了4 - 5倍，而在其余毒株中，神经氨酸酶的免疫原性仅增加了1 - 5倍。
