使用嗜热栖热菌的DNA聚合酶扩增爱泼斯坦-巴尔病毒和人类免疫缺陷病毒的DNA序列。
Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus.
作者信息
Glukhov A I, Gordeev S A, Vinogradov S V, Kiselev V I, Kramarov V M, Kiselev O I, Severin E S
机构信息
Research Center of Molecular Diagnostics, USSR Ministry of Health, Moscow.
出版信息
Mol Cell Probes. 1990 Dec;4(6):435-43. doi: 10.1016/0890-8508(90)90002-h.
Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.
我们使用嗜热栖热菌的嗜热DNA聚合酶，通过聚合酶链反应（PCR）扩增了爱泼斯坦-巴尔病毒（EBV）和人类免疫缺陷病毒（HIV）的特定DNA序列。嗜热栖热菌的DNA聚合酶（分子量为80 - 86 kDa）在物理化学性质上与嗜热水生菌的DNA聚合酶（分子量为62 - 68 kDa）不同。为了扩增EBV的特定DNA序列，使用了针对病毒复制子区域（oriP区域）的寡核苷酸引物。扩增的结果是产生了一个特定的405 bp DNA片段。
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