Manca N, Piacentini E, Gelmi M, Calzavara P, Manganoni M A, Glukhov A, Gargiulo F, De Francesco M, Pirali F, De Panfilis G, Turano A
Institute of Microbiology of Brescia University, Spedali Civili of Brescia, Italy.
J Exp Med. 1994 Nov 1;180(5):1973-8. doi: 10.1084/jem.180.5.1973.
Mycosis fungoides (MF) is a rare form of cutaneous T cell lymphoma suspected of having a viral etiology. As in adult T cell leukemia, the virus involved may be human T lymphotropic virus type 1 (HTLV-1). We cultured the peripheral blood mononuclear cells (PBMC) of 29 patients with MF HTLV-1 seronegative by enzyme-linked immunosorbent assay and Western blot. The presence of reverse transcriptase (RT) and p24 antigen was investigated in the concentrate supernatant of the culture. The DNA of all studied patients was submitted to polymerase chain reaction and Southern blot analysis using primers and probes recognizing the tax region of HTLV-1/2 and the pol region of HTLV-1. 10 of 29 patients were found positive to HTLV-1, whereas they were always negative to RT and p24. The same results were confirmed in double blind after 6 mo. Our findings suggest HTLV-1 may be involved in the etiology of MF, at least in certain cases.
蕈样肉芽肿（MF）是一种罕见的皮肤T细胞淋巴瘤，怀疑有病毒病因。与成人T细胞白血病一样，所涉及的病毒可能是人嗜T淋巴细胞病毒1型（HTLV-1）。我们通过酶联免疫吸附测定和蛋白质印迹法培养了29例HTLV-1血清阴性的MF患者的外周血单个核细胞（PBMC）。在培养物的浓缩上清液中研究逆转录酶（RT）和p24抗原的存在。使用识别HTLV-1/2的tax区域和HTLV-1的pol区域的引物和探针，将所有研究患者的DNA进行聚合酶链反应和Southern印迹分析。29例患者中有10例被发现HTLV-1呈阳性，而他们的RT和p24始终为阴性。6个月后双盲法证实了相同的结果。我们的研究结果表明，HTLV-1可能至少在某些情况下参与了MF的病因。
