Aerni Hans-Rudolf, Cornett Dale S, Caprioli Richard M
Mass Spectrometry Research Center, Department of Chemistry, Vanderbilt University, 465 21st Avenue South, Medical Research Building 3, Room 9160, Nashville, Tennessee 37232-8575, USA.
Anal Chem. 2009 Sep 1;81(17):7490-5. doi: 10.1021/ac900974j.
Analysis of formalin-fixed paraffin-embedded tissues (FFPE) is increasingly recognized as a strategy for the discovery and validation of clinically useful biomarker candidates. Large tissue collections including tissue microarrays (TMAs) are available, but current analytical strategies for their characterization have limited throughput. In this report, we describe a workflow for rapid analysis of hundreds of FFPE tissue specimens. The strategy combines parallel sample processing and on-chip electrophoresis with automated matrix-assisted laser desorption ionzation mass spectrometry (MALDI MS) analysis. The method is optimized for small quantities of clinically valuable tissues allowing detection of hundreds of peptides from a single core in a TMA section. We describe results from the optimization of the method and apply it for the analysis of tissue microarrays containing formalin fixed tissue specimens from human kidney.
福尔马林固定石蜡包埋组织（FFPE）分析日益被视为一种发现和验证具有临床应用价值生物标志物候选物的策略。包括组织微阵列（TMA）在内的大型组织库已经存在，但目前用于其表征的分析策略通量有限。在本报告中，我们描述了一种用于快速分析数百个FFPE组织标本的工作流程。该策略将并行样品处理和芯片电泳与自动基质辅助激光解吸电离质谱（MALDI MS）分析相结合。该方法针对少量具有临床价值的组织进行了优化，可从TMA切片中的单个组织芯检测数百种肽段。我们描述了该方法优化的结果，并将其应用于分析包含来自人肾脏的福尔马林固定组织标本的组织微阵列。
