Casadonte R, Longuespée R, Kriegsmann J, Kriegsmann M
Proteopath GmbH, Trier, Germany.
Proteopath GmbH, Trier, Germany; Institute of Molecular Pathology, Trier, Germany; Center for Histology, Cytology and Molecular Diagnostics, Trier, Germany.
Adv Cancer Res. 2017;134:173-200. doi: 10.1016/bs.acr.2016.11.007. Epub 2017 Jan 12.
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) technology creates a link between the molecular assessment of numerous molecules and the morphological information about their special distribution. The application of MALDI IMS on formalin-fixed paraffin-embedded (FFPE) tissue microarrays (TMAs) is suitable for large-scale discovery analyses. Data acquired from FFPE TMA cancer samples in current research are very promising, and applications for routine diagnostics are under development. With the current rapid advances in both technology and applications, MALDI IMS technology is expected to enter into routine diagnostics soon. This chapter is intended to be comprehensive with respect to all aspects and considerations for the application of MALDI IMS on FFPE cancer TMAs with in-depth notes on technical aspects.
基质辅助激光解吸/电离成像质谱（MALDI IMS）技术在众多分子的分子评估与其特殊分布的形态学信息之间建立了联系。MALDI IMS在福尔马林固定石蜡包埋（FFPE）组织微阵列（TMA）上的应用适用于大规模发现分析。当前研究中从FFPE TMA癌症样本获取的数据非常有前景，常规诊断应用也正在开发中。随着当前技术和应用的迅速发展，预计MALDI IMS技术很快将进入常规诊断。本章旨在全面涵盖MALDI IMS在FFPE癌症TMA上应用的各个方面和注意事项，并对技术方面进行深入说明。
