Matsui K, Arai T
Department of Microbiology, Meiji College of Pharmacy, Tokyo.
Microbiol Immunol. 1990;34(11):917-27. doi: 10.1111/j.1348-0421.1990.tb01070.x.
The protective immunity against Salmonella typhimurium-infection in mice immunized with porins from mutant strains of S. typhimurium was studied. A high level of protection against S. typhimurium infection was achieved in mice immunized with native porins from S. typhimurium LT2 (wild-type strain) but not from S. typhimurium SH6017, SH6260, or SH5551 (mutant strains), which produce 34K, 35K, or 36K porin, respectively. Moreover, when mice were immunized with mixtures of 34K, 35K, and 36K porins (34K + 35K, 35K + 36K, 34K + 36K, or 34K + 35K + 36K porin) or LT2 porin heated at 100 C for 2 min in 2% SDS (heat-denatured LT2 porin), the degree of protective immunities in the mice was very much lower than that in the mice immunized with the native LT2 porin. However, antisera raised against these porins showed no significant differences of the antibody titer against LT2 porin or LT2 whole cells. On the other hand, mice immunized with the native LT2 porin--but not 34K, 35K, 36K, 34K + 35K + 36K, and the heat-denatured LT2 porins--exhibited significant levels of delayed-type hypersensitivity reaction and interleukin-2 production when they were elicited with whole cells of S. typhimurium LT2. These observations suggested that the high level of protection induced by the native LT2 porin immunization was dependent on the induction of cell-mediated immunity.
研究了用鼠伤寒沙门氏菌突变株的孔蛋白免疫的小鼠对鼠伤寒沙门氏菌感染的保护性免疫。用鼠伤寒沙门氏菌LT2（野生型菌株）的天然孔蛋白免疫的小鼠对鼠伤寒沙门氏菌感染具有高水平的保护作用，但用分别产生34K、35K或36K孔蛋白的鼠伤寒沙门氏菌SH6017、SH6260或SH5551（突变株）的天然孔蛋白免疫的小鼠则没有这种保护作用。此外，当用34K、35K和36K孔蛋白的混合物（34K + 35K、35K + 36K、34K + 36K或34K + 35K + 36K孔蛋白）或在2% SDS中于100℃加热2分钟的LT2孔蛋白（热变性LT2孔蛋白）免疫小鼠时，小鼠的保护性免疫程度远低于用天然LT2孔蛋白免疫的小鼠。然而，针对这些孔蛋白产生的抗血清对LT2孔蛋白或LT2全细胞的抗体滴度没有显著差异。另一方面，用天然LT2孔蛋白免疫的小鼠——而不是用34K、35K、36K、34K + 35K + 36K和热变性LT2孔蛋白免疫的小鼠——在用鼠伤寒沙门氏菌LT2全细胞激发时表现出显著水平的迟发型超敏反应和白细胞介素-2产生。这些观察结果表明，天然LT2孔蛋白免疫诱导的高水平保护依赖于细胞介导免疫的诱导。
