Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea.
Korean Institute of Tuberculosis, Korean National Tuberculosis Association, Seoul 137-140, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Feb;60(Pt 2):439-443. doi: 10.1099/ijs.0.012054-0. Epub 2009 Aug 4.
A previously unidentified, slowly growing, scotochromogenic Mycobacterium species, represented by strain 31118(T), was discovered during hsp65 sequence-based reidentification of Korean clinical isolates that had been previously identified as Mycobacterium scrofulaceum by conventional biochemical tests. Although the 16S rRNA gene sequence of strain 31118(T) was identical to that of the recently described Mycobacterium seoulense, phylogenetic analyses based on three independent alternative targets (rpoB, hsp65 and the 16S-23S internal transcribed spacer) showed that it was closely related to M. seoulense but was a distinct phylogenetic entity. Furthermore, the phenetic characteristics of this strain were more similar to those of M. scrofulaceum than to those of M. seoulense. Taken together, these results support the conclusion that this strain represents a novel mycobacterium species, for which the name Mycobacterium paraseoulense sp. nov. is proposed. The type strain is 31118(T) (=DSM 45000(T) =KCTC 19145(T)).
一种以前未被识别的、生长缓慢的、光产色分枝杆菌种，由菌株 31118(T) 代表，在对韩国临床分离株进行 hsp65 序列为基础的重新鉴定时发现，这些分离株之前通过常规生化试验被鉴定为耻垢分枝杆菌。虽然菌株 31118(T) 的 16S rRNA 基因序列与最近描述的汉城分枝杆菌相同，但基于三个独立替代靶标（rpoB、hsp65 和 16S-23S 内部转录间隔区）的系统发育分析表明，它与汉城分枝杆菌密切相关，但却是一个不同的系统发育实体。此外，该菌株的表型特征与耻垢分枝杆菌更相似，而与汉城分枝杆菌不相似。综合这些结果表明，该菌株代表了一种新型分枝杆菌种，建议将其命名为副汉城分枝杆菌。模式菌株为 31118(T)(=DSM 45000(T)=KCTC 19145(T))。
