Balcázar José Luis, Planas Miquel, Pintado José
Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), Eduardo Cabello 6, 36208, Vigo, Spain,
Curr Microbiol. 2014 Sep;69(3):329-33. doi: 10.1007/s00284-014-0588-6. Epub 2014 May 7.
A Gram-positive, aerobic, non-motile, non-sporulating, acid-fast, and rod-shaped bacterium (BFLP-6(T)), previously isolated from a seahorse (Hippocampus guttulatus) with tail rot, was studied using a polyphasic taxonomic approach. Growth occurred at 15-35 °C (optimum 25 °C), at pH 5.0-10.0 (optimum pH 7.0) and at NaCl concentrations between 0 and 6 % (w/v). The G+C content of DNA was 66.7 mol%. The predominant fatty acids were C(18:1) ω9c, C(16:0) and C(16:1) ω6c. A mycolic acid pattern of alpha-mycolates and keto-mycolates was detected. Analysis of concatenated sequences (16S rRNA, rpoB, ssrA and tuf genes), and chemotaxonomic and phenotypic features indicated that strain BFLP-6(T) represents a novel species within the genus Mycobacterium, for which the name Mycobacterium hippocampi sp. nov. is proposed. The type strain is BFLP-6(T) (=DSM 45391(T) =LMG 25372(T)).
一株革兰氏阳性、需氧、不运动、不产芽孢、抗酸且呈杆状的细菌（BFLP-6(T)），先前从一条患有尾部腐烂病的海马（Hippocampus guttulatus）中分离得到，采用多相分类学方法对其进行了研究。该菌在15 - 35 °C（最适温度25 °C）、pH 5.0 - 10.0（最适pH 7.0）以及NaCl浓度为0至6%（w/v）的条件下生长。DNA的G + C含量为66.7 mol%。主要脂肪酸为C(18:1) ω9c、C(16:0)和C(16:1) ω6c。检测到α-分枝菌酸和酮基-分枝菌酸的分枝菌酸模式。对串联序列（16S rRNA、rpoB、ssrA和tuf基因）以及化学分类和表型特征的分析表明，菌株BFLP-6(T)代表分枝杆菌属内的一个新物种，为此提议将其命名为海马分枝杆菌（Mycobacterium hippocampi sp. nov.）。模式菌株为BFLP-6(T)（=DSM 45391(T) =LMG 25372(T)）。
