Quijano Guillermo, Hernandez María, Thalasso Frédéric, Muñoz Raúl, Villaverde Santiago
Departmento de Ingeniería Química y Tecnología del Medio Ambiente, Universidad de Valladolid, Paseo del Prado de Magdalena, s/n, Valladolid, Spain.
Appl Microbiol Biotechnol. 2009 Oct;84(5):829-46. doi: 10.1007/s00253-009-2158-6. Epub 2009 Aug 5.
Two-phase partitioning bioreactors (TPPBs) in environmental biotechnology are based on the addition of a non-aqueous phase (NAP) into a biological process in order to overcome both mass-transfer limitations from the gas to aqueous phase and pollutant-mediated inhibitions. Despite constituting a robust and reliable technology in terms of pollutant biodegradation rates and process stability in wastewater, soil, and gas treatment applications, this superior performance only applies for a restricted number of pollutants or contamination events. Severe limitations such as high energy requirements, high costs of some NAPs, foaming, or pollutant sequestration challenge the full-scale application of this technology. The introduction of solid NAPs into this research field has opened a promising pathway for the future development of TPPBs. Finally, this work reviews fundamental aspects of NAP selection and mass transfer and identifies the niches for future research: low energy-demand bioreactor designs, experimental determination of partial mass transfers, and solid NAP tailoring.
环境生物技术中的两相分配生物反应器（TPPB）是基于向生物过程中添加非水相（NAP），以克服从气相到水相的传质限制以及污染物介导的抑制作用。尽管在废水、土壤和气体处理应用中，就污染物生物降解率和过程稳定性而言，TPPB构成了一项强大且可靠的技术，但这种卓越性能仅适用于有限数量的污染物或污染事件。诸如高能量需求、某些NAP的高成本、起泡或污染物螯合等严重限制因素对该技术的大规模应用构成了挑战。将固体NAP引入这一研究领域为TPPB的未来发展开辟了一条充满希望的途径。最后，这项工作回顾了NAP选择和传质的基本方面，并确定了未来研究的方向：低能量需求的生物反应器设计、部分传质的实验测定以及固体NAP的定制。
