Muñoz Raul, Villaverde Santiago, Guieysse Benoit, Revah Sergio
Valladolid University, Department of Chemical Engineering and Environmental Technology, Paseo del Prado de la Magdalena, s/n, Valladolid, Spain.
Biotechnol Adv. 2007 Jul-Aug;25(4):410-22. doi: 10.1016/j.biotechadv.2007.03.005. Epub 2007 Apr 1.
Two-phase partitioning bioreactors (TPPBs) allow the biological removal of volatile organic compounds (VOCs) from contaminated gas streams at unprecedented rates and concentrations. TPPBs are constructed by adding a non-aqueous phase (e.g. hexadecane, silicone oil) to an aqueous phase that contains the microorganisms responsible for degrading the VOCs. Presence of a water-immiscible phase improves the transfer of hydrophobic substrates (e.g. hexane, oxygen) or reduces the toxicity of inhibitory substances (e.g. benzene, toluene) to the microorganisms present in the aqueous phase. The non-aqueous phase is selected based on cost, safety, good partitioning properties towards the target pollutants, biocompatibility, and non-biodegradability. TPPBs have hitherto been designed as laboratory-scale well-mixed stirred-tank reactors or as biofilters that contain a non-aqueous phase. Scale-up and industrial use of TPPBs require elucidation and modeling of the mechanisms of substrate transfer and uptake; understanding of the mechanisms of microbial selection; identification or synthesis of new inexpensive and robust non-aqueous phases; and generation of suitable guidelines for process design and control.
两相分配生物反应器（TPPB）能够以前所未有的速率和浓度从受污染气流中生物去除挥发性有机化合物（VOC）。TPPB是通过向含有负责降解VOC的微生物的水相中添加非水相（如十六烷、硅油）构建而成。水不混溶相的存在改善了疏水性底物（如己烷、氧气）的传递，或降低了抑制性物质（如苯、甲苯）对水相中微生物的毒性。非水相的选择基于成本、安全性、对目标污染物良好的分配特性、生物相容性和不可生物降解性。迄今为止，TPPB被设计为实验室规模的充分混合搅拌釜式反应器或含有非水相的生物滤池。TPPB的放大和工业应用需要阐明和模拟底物传递与摄取机制；理解微生物选择机制；鉴定或合成新型廉价且稳定的非水相；以及制定合适的工艺设计和控制指南。
