Teanpaisan R, Hintao J, Dahlén G
Department of Stomatology, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.
Anaerobe. 2009 Aug;15(4):160-3. doi: 10.1016/j.anaerobe.2009.01.004.
Although lactobacilli are part of normal oral, gastrointestinal and genitourinary flora, they are an uncommon cause of infections in human. Lactobacillus-associated infections have generally occurred in patients with serious underlying conditions e.g. diabetes and cancer that might favour certain microorganisms. The aim of this study was to characterize species and genotypes of lactobacilli isolated from diabetic patients and non-diabetic subjects. One hundred and five type 2 diabetic patients and 103 non-diabetic subjects were recruited in this study. A total of 170 isolates of Lactobacillus were identified using 16S rRNA gene PCR-RFLP and genotyping were performed using AP-PCR by ERIC primers. It was found that type 2 diabetic patients had a significantly higher prevalence (p = 0.008) and level of lactobacilli than non-diabetic controls (p = 0.030). The most frequently isolated Lactobacillus spp. were L. casei/paracasei and L. fermentum in both the diabetic and non-diabetic groups. Strains of L. casei/paracasei and L. fermentum from between and within individuals were genotyped, and the genotyping of Lactobacillus strains showed diversity between individuals. One up to three genotypes of these two species could be found in the same subject. Interestingly, fewer genotypes were found in the diabetic patients than in the non-diabetic subjects.
尽管乳酸杆菌是正常口腔、胃肠道和泌尿生殖系统菌群的一部分，但它们在人类感染中并不常见。乳酸杆菌相关感染通常发生在患有严重基础疾病（如糖尿病和癌症）的患者中，这些疾病可能有利于某些微生物生长。本研究的目的是鉴定从糖尿病患者和非糖尿病受试者中分离出的乳酸杆菌的种类和基因型。本研究招募了105名2型糖尿病患者和103名非糖尿病受试者。使用16S rRNA基因PCR-RFLP鉴定出总共170株乳酸杆菌分离株，并使用ERIC引物通过AP-PCR进行基因分型。结果发现，2型糖尿病患者的乳酸杆菌患病率（p = 0.008）和水平（p = 0.030）显著高于非糖尿病对照组。在糖尿病组和非糖尿病组中，最常分离出的乳酸杆菌种类是干酪乳杆菌/副干酪乳杆菌和发酵乳杆菌。对个体之间和个体内部的干酪乳杆菌/副干酪乳杆菌和发酵乳杆菌菌株进行了基因分型，乳酸杆菌菌株的基因分型显示个体之间存在差异。在同一受试者中可发现这两个物种的1至3种基因型。有趣的是，糖尿病患者中发现的基因型比非糖尿病受试者中的少。
