Department of Oral Microbiology and Immunology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Clin Exp Dent Res. 2021 Oct;7(5):924-933. doi: 10.1002/cre2.426. Epub 2021 Mar 31.
To determine acid-producing capacity and anti-microbial activity of Lactobacillus species collected pretreatment and post treatment in head and neck cancer patients.
Lactobacillus isolates from 21 patients pretreatment and post treatment were identified using molecular methods. The patients' stimulated salivary secretion was determined pretreatment, and 6 and 12 months post treatment and caries lesions/new filled surfaces registered at 24 months post treatment. The acid-producing capacity of the Lactobacillus isolate was analyzed using a colorimetric fermentation test in microtiter plates. The anti-microbial activity of the isolates against Streptococcus mutans associated with caries, and against the mucosal pathogens Staphylococcus aureus, Candida albicans, and Enterococcus faecalis was analyzed by determining inhibitory zones on agar plates.
The most frequent species were L. paracasei (n = 21), L. casei/rhamnosus (n = 17) and L. fermentum (n = 10). Sixty-seven percent of the patients harbored L. paracasei either at 6 or 12 months post radiotherapy. The corresponding figures for L. casei/rhamnosus and L. fermentum were 62% and 33%. L. paracasei strains showed the best acid-producing capacity and L. fermentum strains the lowest. Strong acid-producing capacity was most common among isolates collected at 6 months post treatment. Seventy-two percent of the strains showed an anti-microbial activity against S. mutans, one strain against S. aureus and none against C. albicans or E. faecalis.
The most frequent species isolated from head and neck cancer patients both pretreatment and post treatment were L. paracasei, L. casei/rhamnosus, and L. fermentum. L. paracasei showed the best acid-producing capacity and the highest proportion with anti-microbial activity against S. mutans.
确定头颈癌患者预处理和后处理时收集的乳杆菌属的产酸能力和抗微生物活性。
使用分子方法鉴定 21 名预处理和后处理患者的乳杆菌分离株。在预处理时测定患者的刺激性唾液分泌量，并在治疗后 6 个月和 12 个月以及治疗后 24 个月时记录龋齿病变/新填充表面。使用微量滴定板比色发酵试验分析乳杆菌分离株的产酸能力。通过确定琼脂平板上对致龋相关链球菌、黏膜病原体金黄色葡萄球菌、白色念珠菌和粪肠球菌的抑制区，分析分离株对这些病原体的抗微生物活性。
最常见的物种是副干酪乳杆菌（n=21）、干酪乳杆菌/鼠李糖乳杆菌（n=17）和发酵乳杆菌（n=10）。67%的患者在放疗后 6 或 12 个月时携带副干酪乳杆菌。干酪乳杆菌/鼠李糖乳杆菌和发酵乳杆菌的相应数字为 62%和 33%。副干酪乳杆菌株表现出最佳的产酸能力，发酵乳杆菌株产酸能力最低。强产酸能力最常见于治疗后 6 个月采集的分离株。72%的菌株对 S. mutans 具有抗微生物活性，1 株对 S. aureus 具有活性，对 C. albicans 或 E. faecalis 均无活性。
头颈癌患者预处理和后处理时分离出的最常见物种是副干酪乳杆菌、干酪乳杆菌/鼠李糖乳杆菌和发酵乳杆菌。副干酪乳杆菌具有最佳的产酸能力，对 S. mutans 的抗微生物活性比例最高。
