Detection of free cytoplasmic circles of transposon Tn9 multimers in Escherichia coli.
作者信息
Sarkari J F, Mahajan S K
机构信息
Molecular Biology & Agriculture Division, Bhabha Atomic Research Centre, Bombay, India.
出版信息
Mol Biol Rep. 1990 Nov;14(4):223-9. doi: 10.1007/BF00429889.
PMID:1965601
Abstract
Extrachromosomal circular DNA molecules consisting of IS1-cat repeats, (IS1-cat)n, were isolated from an E. coli strain harboring nearly 30 copies of tandemly amplified transposon Tn9 located on the chromosome. The DNA 'circles' were characterized by restriction analysis followed by Southern blotting and electron microscopic examination. Their size varied from approximately 5.5 kb to 53 kb.
摘要
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