来自非洲绿猴细胞系的小多分散环状脱氧核糖核酸的分子特征分析。

Molecular characterization of small polydisperse circular deoxyribonucleic acid from an African green monkey cell line.

作者信息

Bertelsen A H, Humayun M Z, Karfopoulos S G, Rush M G

出版信息

Biochemistry. 1982 Apr 27;21(9):2076-85. doi: 10.1021/bi00538a015.

Abstract

Several size classes of small polydisperse circular (spc) DNA from the African green monkey cell line BSC1 have been cloned into the bacterial plasmid pBR322. Analysis of the cloned spc DNA fragments as well as total spc DNA reveals that (a) most or all cloned spc DNAs share homologies with chromosomal sequences, (b) both unique and repetitive chromosomal sequences are represented in spc DNA, (c) the repetitive sequences in spc DNA include two known major repeat families (the alpha and the Alu) as well as a third, as yet unidentified, set of interspersed repetitive sequences, and (d) the alpha-like sequences are present in an oligomeric series of circular DNA molecules within the spc DNA population. The organizational features of repetitive DNA sequence-carrying circles suggest a mechanism for their generation.

摘要

来自非洲绿猴细胞系BSC1的几个大小类别的小多分散环状（spc）DNA已被克隆到细菌质粒pBR322中。对克隆的spc DNA片段以及总spc DNA的分析表明：（a）大多数或所有克隆的spc DNA与染色体序列具有同源性；（b）spc DNA中既包含独特的染色体序列，也包含重复的染色体序列；（c）spc DNA中的重复序列包括两个已知的主要重复家族（α和Alu）以及第三组尚未鉴定的散布重复序列；（d）α样序列存在于spc DNA群体中的环状DNA分子的寡聚系列中。携带重复DNA序列的环状结构的组织特征提示了它们的产生机制。