Luce Coral, Montpetit Shawn, Gangitano David, O'Donnell Patrick
Sam Houston State University, Huntsville, TX 77340, USA.
J Forensic Sci. 2009 Sep;54(5):1046-54. doi: 10.1111/j.1556-4029.2009.01099.x. Epub 2009 Jul 28.
The AmpFlSTR MiniFiler PCR Amplification Kit is designed to genotype degraded and/or inhibited DNA samples when the AmpFlSTR Identifiler PCR Amplification Kit is incapable of generating a complete genetic profile. Validation experiments, following the SWGDAM guidelines, were designed to evaluate the performance of MiniFiler. Data obtained demonstrated that MiniFiler, when used in conjunction with Identifiler, provided an increased ability to obtain genetic profiles from challenged samples. The optimum template range was found to be between 0.2 and 0.6 ng, with 0.3 ng yielding the best results. Full concordance was achieved between the MiniFiler kit and Identifiler kit except in a single case of a null allele at locus D21S11. Numerous instances of severe heterozygous peak imbalance (<50%) were observed in single source samples amplified within the optimum range of input DNA suggesting that caution be taken when attempting to deduce component genotypes in a mixture.
AmpFlSTR MiniFiler PCR扩增试剂盒旨在对降解和/或受抑制的DNA样本进行基因分型，前提是AmpFlSTR Identifiler PCR扩增试剂盒无法生成完整的基因图谱。按照SWGDAM指南设计了验证实验，以评估MiniFiler的性能。所获得的数据表明，MiniFiler与Identifiler联合使用时，从受挑战样本中获取基因图谱的能力有所提高。最佳模板范围为0.2至0.6 ng，其中0.3 ng产生的结果最佳。除了在D21S11基因座出现单个无效等位基因的情况外，MiniFiler试剂盒和Identifiler试剂盒之间实现了完全一致。在输入DNA的最佳范围内扩增的单一样本中，观察到许多严重杂合峰失衡（<50%）的情况，这表明在试图推断混合物中的成分基因型时应谨慎。
