Institut des Sciences du Végétal, CNRS, 91198 Gif sur Yvette, France.
Plant Physiol. 2009 Nov;151(3):1250-63. doi: 10.1104/pp.109.138024. Epub 2009 Aug 5.
We have identified an active Medicago truncatula copia-like retroelement called Medicago RetroElement1-1 (MERE1-1) as an insertion in the symbiotic NSP2 gene. MERE1-1 belongs to a low-copy-number family in the sequenced Medicago genome. These copies are highly related, but only three of them have a complete coding region and polymorphism exists between the long terminal repeats of these different copies. This retroelement family is present in all M. truncatula ecotypes tested but also in other legume species like Lotus japonicus. It is active only during tissue culture in both R108 and Jemalong Medicago accessions and inserts preferentially in genes.
我们鉴定出一个活跃的蒺藜苜蓿 copia 类反转录转座子，命名为蒺藜苜蓿反转录转座子 1-1（MERE1-1），它是共生 NSP2 基因的插入序列。MERE1-1 属于测序蒺藜苜蓿基因组中的低拷贝数家族。这些拷贝高度相关，但只有三个具有完整的编码区，并且这些不同拷贝的长末端重复序列之间存在多态性。该反转录转座子家族存在于所有测试的蒺藜苜蓿生态型中，但也存在于其他豆科植物，如百脉根。它仅在 R108 和 Jemalong 蒺藜苜蓿品系的组织培养中活跃，并且优先插入基因中。
