Kunz J
Institute of Pathology, Potsdam District Hospital, FRG.
Folia Histochem Cytobiol. 1990;28(3):121-8.
Biopsies recovered from human tumors and their preneoplasias (n = 250) were incubated in vitro with 3H-Thymidine, and histoautoradiographic labeling indices (TLIs) were measured. We studied cervical carcinomas and dysplasias of the squamous epithelium of the cervical portio: oral carcinomas and leukoplakias; breast carcinomas and fibrocystic mastopathies; basal cell epitheliomas as well as cerebral tumors of differing degree of malignancy. In each case 2000 to 4000 cells were counted. The relevance of the procedure was examined in methodological investigations. The most important results can be summarized as follows: TLIs represent a relative measure of the size of S-phase fraction of malignant tumors and their behaviour runs parallel with different rate growth. Even for tumors of the same histologic type (Squamous cell carcinoma of the cervix uteri: invasive-ductal breast carcinoma) TLIs are proliferation-kinetic parameters of prognostic significance, in that they characterize the different degrees of malignancy of such tumors. If account is taken of certain conditions, the reliability of the indices is not greatly affected by the intratumoral heterogeneity of cell proliferation. TLIs are more reliable than is histologic description in determining the proliferative potential of dysplasic epithelial changes and various forms of malignancy.
从人类肿瘤及其癌前病变（n = 250）获取的活检组织在体外与³H - 胸腺嘧啶一起孵育，并测量组织放射自显影标记指数（TLI）。我们研究了宫颈阴道部鳞状上皮的宫颈癌和发育异常；口腔癌和白斑；乳腺癌和纤维囊性乳腺病；基底细胞上皮瘤以及不同恶性程度的脑肿瘤。每种情况均计数2000至4000个细胞。在方法学研究中检验了该程序的相关性。最重要的结果可总结如下：TLI代表恶性肿瘤S期部分大小的相对度量，其变化与不同的生长速率平行。即使对于相同组织学类型的肿瘤（子宫颈鳞状细胞癌：浸润性导管乳腺癌），TLI也是具有预后意义的增殖动力学参数，因为它们表征了此类肿瘤的不同恶性程度。如果考虑某些条件，指数的可靠性不会受到肿瘤内细胞增殖异质性的太大影响。在确定发育异常上皮变化和各种恶性形式的增殖潜力方面，TLI比组织学描述更可靠。
